Pole-mutant animals predominantly had nodal lymphomas and histiocytic sarcomas, whereas Pold1 mutants had thymic lymphomas and skin papillomas/sarcomas. Both types of mice had intestinal adenomas (more in Pole) and lung tumors (more in Pold1). Double ABT-737 ic50 knockout animals died early from thymic lymphoma. Spontaneous mutations frequencies were higher in Pole mutants than Pold1 mutants

[ 20••]. One explanation could be that the fidelity of lagging strand replication is greater than that of leading strand, because post-replicative DNA mismatch repair (MMR) preferentially corrects lagging strand replication errors [ 21 and 22]. However, this in contrast with the data from yeast [ 14]. Genetic studies in proofreading-deficient, haploid yeast strains which also carried a MMR-defect showed a synthetically lethal phenotype indicating a synergistic effect on the mutation rate of proofreading and MMR [ 23 and 24]. This was also confirmed in mouse

studies where loss of both proofreading and MMR led to embryonic lethality [ 20•• and 25]. Conversely, others have speculated that MMR deficiency may be required for the EDM mutator phenotype to be manifested [ 26]. Even if replication fidelity is high, some errors always escape proofreading and are then corrected by MMR [27]. In studies beginning in the late 1980s, it was found that germline mutations in four MMR genes (MSH2, MLH1, MSH6 and PMS2) were causative for the hereditary colorectal and other cancers that are present in Lynch syndrome (reviewed in Selleck ZD1839 [ 28 and 29]). Furthermore, somatic silencing of MLH1 expression occurs in Clomifene several cancer types, notably CRC and endometrial cancer (EC). In addition, bi-allelic germline MUTYH mutations predispose to adenomatous colorectal polyposis and CRC through defective base excision repair. We recently identified specific germline EDMs in POLD1 and POLE that are causative for the development of multiple colorectal adenomas and CRC. Since the phenotype overlaps with those who carry germline mutations in MUTYH and the MMR genes, we have called the disease PPAP [ 30 and 31••]. Using a combination of whole-genome sequencing of highly selected multiple adenoma

patients, linkage analysis, and studies of loss-of-heterozygosity (LOH) in tumors, followed by replication in a large set of familial CRC cases [31••] we identified one germline mutation in POLE (p.Leu424Val) and one in POLD1 (p.Ser478Asn) that were not present in nearly 7000 UK controls or in public databases of controls. In addition, another probably pathogenic mutation, POLD1 p.Pro327Leu, was found in a further patient with multiple adenomas. Patients who carry EDMs in POLE or POLD1 show variable phenotypes: some have tens of adenomas that do not appear to progress rapidly to cancer, whereas others have a small number of large adenomas or early-onset carcinomas, thus resembling Lynch syndrome. Interestingly, female carriers of POLD1 p.Ser478Asn have a greatly increased risk of EC.