Nonetheless, electrospinning has limitations and hazards, negating its wider exploration, for example, the inability to handle highly conducting suspensions, to its hazardous high voltage. Hence, to date electrospinning has undergone an exhaustive research regime to a point of clicheacute. Thus, in the

work reported herein we unveil a competing technique to electrospinning, which has overcome the above limitations and hazards yet comparable in capabilities. The fiber preparation approach unearthed herein is referred to as “”pressure driven spinning (PDS).”" BX-795 The driving mechanism exploited in this fiber spinning process is the pressurized by-pass flow. This mechanism allows the drawing of either

micro- or PD98059 concentration nanosized fibers while processing polymeric suspensions containing a wide range of advanced materials spanning structural, functional, and biological entities. Similar to electrospinning if the collection time of these continuous formed fibers is varied, composite scaffolds and membranes are generated. In keeping with our interests, multicompositional structural entities such as these could have several applications in biology and medicine, for example, ranging from the development of three-dimensional cultures (including disease models) to the development of synthetic tissues and organ structures to advanced approaches for controlled and targeted therapeutics.”
“Bio-electrospraying and aerodynamically assisted bio-jetting are two direct cell handling approaches recently pioneered,

which have demonstrated significant applicability to the life sciences. These two bioprotocols have undergone scientific rigor, which have seen these techniques been SBE-β-CD mouse explored in conjunction with a wide range of immortalized, primary and stem cells, and those whole organisms. Those studies have demonstrated a cellular population of >70% viable post-treatment in comparison with controls. Although, these studies assessed cellular viability, cell surface molecules play a critical role in several cellular functions, in particular, have importance to tissue engineering and regenerative medicine. Thus, in the studies reported herein, we demonstrate post-treated viable cells retain their cell surface marker expression levels in comparison to controls, over both short and long time points. Therefore, these studies further push back the frontiers of both bio-electrosprays and aerodynamically assisted bio-jetting in their endeavor as novel strategies for tissue engineering and regenerative biology/medicine with possible targeted clinical utility.”
“Narcolepsycataplexy syndrome is characterized by excessive daytime sleepiness, cataplexy, sleep paralysis, hypnagogic hallucinations and disturbed nocturnal sleep. It is strongly associated with the genetic marker, human leucocyte antigen (HLA) DQB1*06:02.