Data collection The number of falls during the set of specified exercises was counted in order to assess the level of fatigue and its influence on their performance. For the CG group, blood glucose (Accu-check active Roche®) and Lactate (Accutrend Lactate, Roche®)was measured on three moments–before the warm up (REST), before the beam balance set (PRE SERIES) and immediately after the set (POS SETS). For the FG group, blood glucose and Lactate was measured during four moments–before the fatigue circuit (REST), before the warm up and after the fatigue (FATIGUE), before the beam balance set (PRE SETS), and immediately after the set (POS SETS). Experimental

design On both experimental days, WATER DAY and CARBOHYDRATE DAY, we counted the number of falls during the sets on the balance beam, measured blood glucose

Caspase inhibitor and lactate in three moments: rest, before the sets and after the sets. For the fatigue group, we also measured blood glucose and lactate right after the fatigue circuit (Table 1). Table 1 Scheme of the experimental design Experimental days/Groups CG FG WATER DAY (DAY 1) Rest Rest     20 minute fatigue   10 min Warm up 10 min Warm up   5 sets 5 sets CARBOHYDRATE Selleckchem HDAC inhibitor DAY (DAY 2) Rest Rest   20 minute fatigue Flavored Juice Maltodextrin 10 min warm up 10 min warm up   5 sets 5 sets Statistical analysis We used a two way ANOVA analysis, considering fatigue and supplementation as variables, and used independent Student T test to investigate differences between the groups diglyceride when observed as pairs. Results were displayed as mean ± se (mean ± standard error) and significance level was set to p < 0.05. Results and discussion The glucose and lactate profile on REST was similar

to both groups on both days (WATER DAY–glucose 97.0 ± 15.5 mg/dl for CG and 97.2 ± 16.7 mg/dl for FG p = 0.98). Lactate 1.6 ± 0.4 mmol/L for CG and 1.7 ± 0.3 mmol/L for FG p = 0.67); (CARBOHYDRATE DAY–glucose 94.5 ± 18.0 mg/dl for CG and 88.0 ± 8.2 mg/dl for FG p = 0.48; Lactate 1.2 ±0.4 mmol/L for CG and 1.4 ± 0.2 mmol/L for FG p = 0.19). The fatigue protocol was efficient, showed by a significant increase on lactate and blood glucose concentration to FG on FATIGUE (after the fatigue circuit) on both days comparing to REST (WATER DAY–lactate 13.92 ± 1.48 mmol/L FATIGUE and 1.17 ± 0.42 mmol/L REST p = 0.00007 glucose 118 ± 39.07 mg/dl FATIGUE and 97.2 ± 16.72 mg/dl REST p = 0.12); (CARBOHYDRATE DAY–lactate 10.2 ± 3.0 mmol/L FATIGUE and 1.4 ± 0.2 mmol/L REST p = 0.00007 glucose 112.0 ± 11.44 mg/dl FATIGUE and 88.0 ± 8.25 mg/dl REST p = 0.0007). The increase in glucose concentration with consequent lactate production is a response to the high intensity exercise represented by the fatigue protocol, as seen in some classic studies [15–17].