Transcribed ssRNA molecules were mixed in precise equimolar amounts. This dsRNA was adjusted to 7.2 × 107 copies/μl. Serial ten-fold dilutions of the standard RNA were included in each Compound Library assay. Cycle Threshold (Ct) values were plotted against the serial dilutions of the standard RNA to produce the standard curve to determine the genome copies per ml of blood

sample. All horses were sero-negative at the beginning of the study and developed serum VNAb upon inoculation with MVA-VP2(9). No adverse reactions to vaccination were seen, other than a transient inflammation at the injection site which subdued after 24 h. On day 34 of the study, the vaccinated horses and 3 unvaccinated controls were challenged with AHSV-9. Following challenge with AHSV-9, all vaccinated animals remained clinically normal and their rectal temperatures remained within physiological ranges until the end of the study (Fig. 1). In contrast, all the control horses developed clinical signs consistent with the cardiac form of African horse sickness. They became febrile by day 2 post-infection as rectal temperatures reached values ranging between 39.08 to 39.28, a significant rise compared with the vaccinated group (Wilcoxon rank sum test: P = 0.05). These temperatures

peaked on day 3 (horse C3) and day 4 (horses C1 and C2), and then declined in the hours before death. Clinical signs in BIBW2992 concentration the control animals were present by day 3 post-infection and comprised: mild general malaise and depression; palpebral oedema and conjunctivitis; and mild nasal Cediranib (AZD2171) discharges. These clinical signs slightly worsened

on day 4 and progressed very rapidly thereafter. The three control horses died between the end of day 5 (C3) and day 6 (C1 and C2). The post-mortem lesions of control horses were consistent with the cardiac form of AHS, and included: oedema, congestion and haemorrhages of the ocular conjunctiva; the presence of a yellow gelatinous oedema in the inter-muscular fasciae of the neck and sub-scapular region, oesophagus and epicardial surfaces; hydropericardium; hydrothorax; sub-endocardial haemorrhages; and congestion of the kidneys, liver, spleen and stomach mucosa. The lungs presented mildly enlarged interlobular septi but the typical frothy fluid of the ‘pulmonary form’ of AHS was not present. The results of these tests are presented in Table 1 and Table 2. All vaccinated animals were negative for infectious virus in blood whereas the control horses developed viraemia with viral titres that ranged between 104.5 to 104.6 TCID50/ml on day 3, and between 105.5 to 105.8 TCID50/ml on day 5. The differences between vaccinates and controls on each day were statistically significant (Wilcoxon rank-sum test: P = 0.03 for both days) Real time RT-PCR results indicate that there were significant differences in the viral load between vaccinates and controls. The mean viral RNA log10 copy number on day 3 was 106.8 for controls and 102.