Unfortunately challenge experiments could not be performed in guinea pigs, as horses are the natural host for AHSV. The AHSV infection model using interferon-α knockout mice were recently reported [17]. The use of the small animal model for our future VP2 vaccine study should help to evaluate the vaccine efficacy. Cross-reactive Abs to genetically related AHSV serotypes were shown by IPMA with lower Ab titers than serotype

specific reactions, except for AHSV-5 and AHSV-8, in which α-AHSV-5 VP2 serum reacted strongly to both AHSV-5 and Anti-cancer Compound Library high throughput AHSV-8, and vice versa. Interestingly, no cross neutralization Abs between AHSV-5 and AHSV-8 were detected. It would be thought that more antibodies to non-neutralizing than to neutralizing domains of AHSV-5 and AHSV-8 VP2 were elicited. These variations in the feasibility of eliciting non-neutralizing Abs and nAbs between serotypes could contribute the considerable differences in the nAb titers. Although the crystal structure of AHSV VP2 has not been solved, neutralizing domains on the secondary structure containing amino acid 199–689 of VP2 were demonstrated [34]. To avoid

eliciting non-neutralizing Abs, expression and immunization of only neutralization domain of VP2 may help to induce nAbs more efficiently. In contrast to AHSV-5 and -8, VP2 of AHSV serotype 9 induced nAbs against serotype Roxadustat solubility dmso 6 (nAb titer of 12 with 95% CI: 3–21) which was not detectable by IPMA, suggesting that the non-nAb is not necessarily higher than nAb. This phenomenon is probably due to the structural similarity and dissimilarity between VP2s of relevant serotypes. Here, we have also studied two cocktails of four or five tuclazepam VP2 proteins. The results suggested a dose-dependent immune

response, since all serotype specific nAb titers were lower after immunization with cocktails of VP2 proteins (10/12.5 μg of each VP2 per animal) than those with individual VP2 immunization (50 μg of VP2 per animal). However, this reduction was not linearly related to the amount of injected VP2. The reduction of 4–5 fold VP2 protein in cocktails resulted in 4 to 40 fold reduced nAb titers compared to single VP2 immunization; e.g. for serotype 5, 179 by single and 53 by cocktail VP2 (±30% difference), and for serotype 9, 853 by single and 19 by cocktail VP2 (±2% difference). This might suggest a negative interference between some of the VP2 proteins in cocktails to induce nAbs. The lower serotype specific nAb titer after immunization with cocktails of VP2 proteins could also be due to the simultaneous presentation of various serotype specific epitopes to the immune system or due to the immunodominance of certain serotype specific epitopes. Thus, formulation of VP2 cocktails to protect horses against all included serotypes is also complicated by differences in immunogenicity and possible interference between VP2 proteins to induce humoral immune responses.